flamingotm fluorescent protein gel stain  (Bio-Rad)

Journal: Materials Today Bio

Article Title: Graphdiyne-Ivy fiber neural scaffold promotes stem cell directed differentiation and neuronal maturation

doi: 10.1016/j.mtbio.2026.103022

Figure Lengend Snippet: Material characterization and biocompatibility assessment of GDY-Ivy Fiber Scaffold. (A) SEM characterization: Surface morphology and local magnification of PR, PO, and POGDY scaffolds. (B) SEM image of GDY. (C) SEM-EDS elemental mapping: Distribution of C and O elements and corresponding energy spectrum analysis of the conductive scaffolds for PR, PO, and POGDY groups. (D) FTIR spectra of PO, GDY, and POGDY samples. (E) XPS spectra: High-resolution C 1s spectra of PO, GDY, and POGDY samples. (F) Data analysis of water contact angles for PR, PO, and POGDY fiber membranes, n = 5, one-way ANOVA. (G) Contact angle images of the samples, GDY0.5 specifically labeled as POGDY. (H) Live/dead cell staining of neural stem cells on the fiber conductive scaffolds from each group. (I) Quantitative analysis of cell viability for each group, n = 5. (J) CCK-8 assay: Cell viability trend at 1, 3, and 5 days of culture in different groups, two-way ANOVA. ns, not significant, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, n = 5.

Article Snippet: Cytotoxicity was assessed via Live/Dead double fluorescence staining (Servicebio, China), with live cells stained green and dead cells stained red.

Techniques: Labeling, Staining, CCK-8 Assay

Journal: Cell Reports Methods

Article Title: Multiscale domain identification for spatial transcriptomics via persistent homology

doi: 10.1016/j.crmeth.2026.101376

Figure Lengend Snippet: PHD-MS analyzes tumor microenvironment morphology (A) Expert annotated tumors from immunofluorescent image, with anti-CD3 intensity in pink and DAPI intensity in green. IDC is outlined in yellow, carcinoma in situ is outlined in blue, benign hyperplasia is outlined in red, and unclassified tumor is in green. (B) Prominent multiscale domains identified by PHD-MS, selected from the most persistent domains. Regions are numbered to correspond with the labels in (A). (C) PHD-MS heterogeneity map highlights highly heterogeneous regions. The heterogeneity score approximates the number of persistent domains that contain each spot. (D) Mean normalized immune expression from a set of immune genes (PTPRC, CD4, CD8A, CD14, CD68, and IGHG3). (E) Normalized expression of key oncogenes APOE, PGK1, and MALAT1 in the lower IDC. From DGE analysis, APOE is overexpressed in region 7 relative to 8 and 9, PGK1 is overexpressed in 8, and MALAT1 is overexpressed in 9.

Article Snippet: Visium Invasive Ductal Carcinoma Stained With Fluorescent CD3 Antibody , Zhao et al. , https://www.10xgenomics.com/datasets/invasive-ductal-carcinoma-stained-with-fluorescent-cd-3-antibody-1-standard-1-2-0.

Techniques: In Situ, Expressing